Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shenjian Ai
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 10:01:58
CsgA-EGF
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2152002_sequence (Version 1) |
Description
Through molecular programming of the bacterial extracellular matrix material by genetically appending EGF peptide to the amyloid protein CsgA, the dominant proteinaceous component in E. coli biofilms. These engineered CsgA fusion proteins are successfully secreted and extracellularly self-assemble into amyloid nanofibrer networks that retain the functions of the displayed peptide domains. It may be able to use the function of EGF to treat IBD.Notes
We use overlap PCR to connect CsgA and EGF with a linker. And to make a standard part, we performed site-directed mutagenesis to eliminate the restriction site of PstⅠ.Source
For CsgA, it is from a gift from Allen Y. Chen, Biophysics Program, Harvard University.For EGF, it is synthesized by GenScript.