Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: XULIN GE
Date created: 2016-10-12 11:00:00
Date modified: 2016-10-13 08:13:45
Bacteriophage Phi X 174 lysis gene E with T7 and RBS
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K2152004_sequence (Version 1) |
Description
Lysis protein E spanning the inner and outer membrane of the bacterial, leading to low local degradations of peptidoglycan, allow the release of cytoplasmic content.Use IPTG to induce the transcription of T7 promoter,and the expression of gene E protein in E.coli BL21.
Notes
Introduce a Point mutation at a XbaI restriction site.Source
Bacteriophage Phi X 174| Sequence Annotation | Location | Component / Role(s) |
| T7 promoter Ribosome Binding Site Bacteriophage Phi X 174 lysis gene E | 1,17 69,74 82,360 | promoter feature/promoter ribosome_entry_site feature/rbs CDS feature/protein |