BBa_K215250

BBa_K215250 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K215250
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Alex Leone
Date created: 2009-09-29 11:00:00
Date modified: 2015-05-08 01:11:30

Lpp + 1tmr OmpA + GS Linker + TEV site + NheI Site



Types
DnaRegion

Roles
Coding

CDS

Sequences BBa_K215250_sequence (Version 1)

Description

This is a fusion protein that displays your favorite gene inserted into the NheI site.

Notes

Anything inserted into the NheI shouldn't have a start codon and gene should still be in frame.

Source

Harvard iGEM 2006: BBa_J36836. We added a GS Linker and TEV site.

Sequence Annotation Location Component / Role(s)
BBa_J36835 - Lpp Signaling Peptide
BBa_J36836 - 1tmr OmpA
GS Linker - GGGSGGGSGGG
TEV Site - ENLYFQG
NheI
1,87
94,156
157,189
190,210
211,216
feature/BioBrick engineered_region
engineered_region feature/BioBrick
sequence_feature feature/misc
feature/misc sequence_feature
feature/misc sequence_feature
igem#experience
None
 
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K215250/1