Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Edinburgh iGEM 2009
Date created: 2009-10-15 11:00:00
Date modified: 2015-05-08 01:11:30
TNT receptor TNT-R1 + hybrid signal transduction protein Trz
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K216013_sequence (Version 1) |
Description
This consists of the coding sequences for TNT receptor TNT-R1 (made by rational redesign of ribose binding protein by the Hellinga et al, Nature 2003) and the hybrid signal transduction protein Trz (consisting of the extracellular part of chemotaxis receptor Trg fused to the intracellular part of sensor kinase EnvZ, which phosphorylates OmpR, controlling promoters such as PompC; made by Hazelbauer et al).Notes
No special considerations. Note that due to a minor miscalculation, this construct lacks a NotI site in the suffix, but the suffix is otherwise intact; thus it does not fully conform to the standard RFC10, though it can be used in assembly in the usual way. We apologize for this situation and plan to submit a revised version with the missing NotI site, or, alternatively, propose a new standard similar to RFC10 but which does not include the NotI sites in the prefix and suffix.Source
Made by joining BBa_K216004 to BBa_K216002.| Access | Instance | Definition |
| public public | BBa_K216002 BBa_K216004 | BBa_K216002 BBa_K216004 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K216002 BBa_K216004 | 1,851 860,2364 | BBa_K216002 BBa_K216004 |