BBa_K2163000

BBa_K2163000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2163000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Rhushikesh Phadke

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 02:38:24

Measurement of Protease Activity using FRET

Details

• Types: DnaRegion
• Roles: Measurement; engineered_region
• Sequences: BBa_K2163000_sequence (Version 1)

Description

This part Consists of 3 biobricks, namely Cyan Fluorescent Protein, HIV Cleavage Site and Yellow Fluorescent Protein. The cleavage site is specific to HIV 1 Aspartyl Protease. The two proteins form a FRET pair. FRET stands for Fluorescence Resonance Energy Transfer. It is a phenomenon is which excitation of one protein leads to emission from the other, due to overlapping wavelengths.
The two proteins need to be close together for this to take place. In our project we make use of this to measure the protease activity of HIV 1 Aspartyl Protease.

Notes

The design considerations include
1. Choice of the Fluorescence proteins
2. Distance between them

Source

All the biobricks used come from the registry

Components

Access	Instance	Definition
public	BBa_E0020	ecfp
public	BBa_I712015	BBa_I712015
public	BBa_K592101	YFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_E0020	1,723	ecfp
BBa_I712015	732,773	BBa_I712015
BBa_K592101	780,1499	YFP

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2163000/1

Member of these Collections

• iGEM Parts Registry