BBa_K2165001

BBa_K2165001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2165001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Roya Amini-Naieni

Date created: 2016-10-13 11:00:00

Date modified: 2016-10-14 08:35:32

Violacein D gene codon-optimized for S. Cerevisiae

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K2165001_sequence (Version 1)

Description

This​ ​part​ ​is​ ​a​ ​codon​ ​optimized​ ​version​ ​for​ ​yeast​ ​of​ ​the​ ​violacein​ ​D (vioD)​ ​gene​ ​submitted​ ​by​ ​the​ ​Alberta team​ ​in​ ​2013​ ​as​ ​http://parts.igem.org/Part:BBa_K1161004 K1161004 and is easy to insert in a custom violacein system.​ ​VioD ​is​ ​one​ ​of​ ​five​ ​genes​ ​in the violacein metabollic pathway that​ ​together​ ​produce​ ​the​ ​purple​ ​pigmented​ ​antibiotic, violacein,​ ​when​ ​expressed​ ​in​ ​yeast.

Notes

The online codon-optimization tool offered by Integrated DNA Technologies (https://www.idtdna.com/CodonOpt) was used to codon-optimize this sequence for use in yeast. Combinations containing RFC 10 illegal restriction sites were actively avoided.

Source

​VioC​ ​is​ ​originally​ ​from​ ​the​ violacein​ ​metabolic pathway​ ​in​ ​Chromobacterium​ ​Violaceum​, a type of Proteobacteria. The original vioC sequence for the bacteria chassis was submitted by the Alberta team in 2013 as http://parts.igem.org/Part:BBa_K1161004 K1161004 and has been codon-optimized for use in yeast in this part.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
VioD	1,1128	feature/cds, CDS

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2165001/1

Member of these Collections

• iGEM Parts Registry