Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Roya Amini-Naieni
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-14 09:20:00
CUP1 yeast inducible promoter with RFC[10] restriction sites removed
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K2165004_sequence (Version 1) |
Description
CUP1 is a commonly used inducible transcription promoter for yeast that leads to enhanced expression of its respective gene(s) in the presence of cupric ions. The documented natural variants of the CUP1 promoter, including part K945002 in the registry (http://parts.igem.org/Part:BBa_K945002), contain RFC[10] illegal restriction sites that have been eliminated in this part so that it may be used in other biobricks.Notes
The Yeast Promoter Atlas was used to prevent altering bases that served a significant regulatory purpose (http://ypa.csbb.ntu.edu.tw/do?act=gene_by_kw&query=YHR053C#promoter_map). One base pair was substituted to eliminate the XbaI site in the middle of the sequence.Source
The CUP1 promoter was identified in the 5' untranslated region of the metallothionein gene in Saccharomyces cerevisiae.| Sequence Annotation | Location | Component / Role(s) |
| pCup1 ChangedBase(GtoA) | 1,263 84,84 | feature/promoter promoter feature/mutation sequence_alteration |