Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Filip Cierniak
Date created: 2016-10-13 11:00:00
Date modified: 2016-10-28 01:03:02
ZnO binding Curli protein
| Types | DnaRegion |
| Roles | Composite engineered_region |
| Sequences | BBa_K2169001_sequence (Version 1) |
Description
Curli subunit CsgA modified with a ZnO binding tag to enable binding of ZnO surfaces.Notes
CsgA is secreted. In order not to interfere with the secretion signal, we had a look at CsgA fusion proteins. C-terminal fusions have been shown to be functional. For cloning into our expression vector with EcoRI, we had to add an RBS before the start codon.Source
CsgA: E. coli genome/ parts registry| Access | Instance | Definition |
| public public public public | BBa_K1650034 BBa_K1650041 BBa_K2169139 BBa_K2169137 | BBa_K1650034 BBa_K1650041 BBa_K2169139 BBa_K2169137 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K1650034 BBa_K1650041 BBa_K2169139 BBa_K2169137 | 1,12 19,471 480,500 509,580 | BBa_K1650034 BBa_K1650041 BBa_K2169139 BBa_K2169137 |