Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: michael ellison
Date created: 2016-10-20 11:00:00
Date modified: 2016-10-29 02:22:04
Chlorite Dismutase CDS+RBS from I. dechloratans with His-tag
| Types | DnaRegion |
| Roles | Translational_Unit engineered_region |
| Sequences | BBa_K2179003_sequence (Version 1) |
Description
This an engineer derivative of the Chlorite dismutase coding sequence from the soil bacterium Ideonella Deschloratans (Thorell et al. 2003). This enzyme coverts chlorite (ClO2) to oxygen and chloride. The 5' sequence that encodes the periplasmic localization signal has been removed. In addition, a His10x tag has been appended to the c-terminus. An internal BsaI site was eliminated by a a silent C-to-G substution at the third codon position at position 198 of the protein coding sequence. The CDS ends with the. consequetiv codons two stop codons TGA. The part is also flanked at either end by two BsaI sites at either end that are internal to the prefix and suffix of pSB1C3. These sites produce non-palindromic sticky end that are differ from one in sequence. They can be useful for the precise unidirectional ligation of a single gene copy to an an appropriately designed part or plasmid backbone. The CDS is preceded with a strong RBS derived form the the expression plasmid PCB-38-441 which is commercially available from DNA 2.0.Thorell, H.D, Karlsson, J., Portelius, E.and Nilsson, T. Biochimica et Biophysica Acta 1577 (2002) 445???451
Notes
see aboveSource
Derived from the soil bacterium Ideonella deschloratans| Sequence Annotation | Location | Component / Role(s) |
| rbs chlorite dismutase BsaI BsaI His Tag GGHHHHHHHHHH | 11,22 27,800 6,11 807,813 765,800 | ribosome_entry_site feature/rbs CDS feature/cds sequence_feature feature/misc feature/misc sequence_feature sequence_feature feature/misc |