Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sofiane Safi-Stibler
Date created: 2016-07-31 11:00:00
Date modified: 2016-08-01 03:23:47
Sucrose Operator - DegU Binding Site
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K2180003_sequence (Version 1) |
Description
We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. The regulatory region is composed by two DegU binding (one forward, one reverse) sites wich are derepressed in the presence of sucrose.Source
The sequence of the regulatory region comes from this site : http://www.prodoric.de/site.php?site_acc=SI002871| Sequence Annotation | Location | Component / Role(s) |
| SacII Restriction Site DegU Binding Site 1 DegU Binding SIte 2 PacI Restriction Site | 1,6 7,14 17,24 25,32 | feature/misc sequence_feature non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/misc sequence_feature |