Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sofiane Safi-Stibler
Date created: 2016-08-04 11:00:00
Date modified: 2016-10-13 10:58:17
PLacO - Promoter LacO for B. subtilis
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K2180006_sequence (Version 1) |
Description
This part is made of PlepA promoter, two LacI binding sites and a RBS. There is a PacI restriction site before LacI binding sites and a SacII restriction site after them. They allows us to remplace the LacI binding sites by another operating sequence. A SalI restriction sites is presents before the PlepA sequence that may be used to insert the whole sequence. We use this part in our project to promote the pigment expression under the control of LacI.Source
The part is made of a previous IGEM part (add part)| Sequence Annotation | Location | Component / Role(s) |
| SalI restriction site PlepA PacI restriction site LacI Operator O1 LacI Operator O1 SacII restriction site RBS NdeI | 10,15 23,179 180,187 188,208 209,229 230,235 236,246 254,258 | sequence_feature feature/misc promoter feature/promoter sequence_feature feature/misc feature/operator operator feature/operator operator sequence_feature feature/misc feature/rbs ribosome_entry_site sequence_feature feature/misc |