BBa_K2180007

BBa_K2180007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2180007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sofiane Safi-Stibler
Date created: 2016-08-04 11:00:00
Date modified: 2016-10-13 10:58:33

PFur - Promoter Fur for B. subtilis



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K2180007_sequence (Version 1)

Description

This part is made of PleiaG promoter, a fur (Ferric Uptake Regulator) binding sites and a RBS. There is a ClaI restriction site before the Fur Box (Fur Binding Site) and a PscI restriction site after. They allows us to remplace Fur Box by another operating sequence. A BamHI restriction sites is present before the PliaG sequence that may be used to insert the whole sequence. We use this part in our project to promote the LacI + LVA expression under the control of Fur.

Notes

We had to check the compatibility of the promoter with the Fur Box.

Source

This part is based on a previous IGEM part (add part). We add the Fur Box site we found (add site)

Sequence Annotation Location Component / Role(s)
BamHI
PliaG
ClaI
Fur Box
PscI
RBS
NcoI
1,6
16,136
137,142
143,163
164,169
170,180
188,193
feature/misc sequence_feature
feature/promoter promoter
sequence_feature feature/misc
feature/binding non_covalent_binding_site
sequence_feature feature/misc
ribosome_entry_site feature/rbs
sequence_feature feature/misc
igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2180007/1