BBa_K2180011

BBa_K2180011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2180011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sofiane Safi-Stibler

Date created: 2016-08-20 11:00:00

Date modified: 2016-10-13 11:22:04

Arsenic Operator - ArsR Binding Site

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K2180011_sequence (Version 1)

Description

We add two restriction sites. SacII before and PacI after the regulatory region in order to use it in our project. They allow us to replace a regulatory region with another regulatory region. This Region allow ArsR to bind to DNA and repress transcription. In the presence of Heavy Metals such as Arsenic or Cadmium transcription is derepressed because of the fixation to ArsR.
Other metalic ions may bind to ArsR.

Source

Biobrick based on a previous biobrick made by the iGEM09_Newcastle Team (2009-10-14) :
http://parts.igem.org/Part:BBa_K174015

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
SacII	1,6	sequence_feature, feature/misc
ArsR Binding Site	7,32	feature/binding, non_covalent_binding_site
ArsR Binding Site	33,58	feature/binding, non_covalent_binding_site
PacI	59,66	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2180011/1

Member of these Collections

• iGEM Parts Registry