BBa_K2184003

BBa_K2184003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K2184003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Margalit Mualem

Date created: 2016-09-18 11:00:00

Date modified: 2016-09-19 08:32:59

Guid RNA for NaCl & Sour flavor

Details

• Types: DnaRegion
• Roles: Protein_Domain; polypeptide_domain
• Sequences: BBa_K2184003_sequence (Version 1)

Description

Identification of the selected allele for a specific taste receptor encoded and sliced in vitro using the Cas9 enzyme. This change will allow us to control and navigate the ability of the sense of taste as needed.

Cas9 is the endonuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. Binding specificity is based on the gRNA sequence and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence.

Notes

This molecule was specially designed to be able to identify SNP in NaCl & Sour flavor and mediates CRISPR editing of the non-taste alleles only

Source

AGCTTTCATGACACACACGA

Other Properties

• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K2184003/1

Member of these Collections

• iGEM Parts Registry