Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marissa Sumathipala
Date created: 2016-09-04 11:00:00
Date modified: 2016-09-15 01:58:44
B.cereus beta amylase w/ no promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K2185005_sequence (Version 1) |
Description
This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the bacterium B.cereus that codes for a beta amylase enzyme. This composite part contains the coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. It does not include a promoter. The amylase enzyme can be expressed by cloning this part into a plasmid with a promoter built in (such as the S.cerevisiae yeast vector pAG36).Notes
Codon optimized for S.cerevisiae, Kpn and Spe sites removed.Source
It is a composite part, coding sequence is sourced from the B.cereus genome.| Access | Instance | Definition |
| public public public public | BBa_K165002 BBa_K792002 BBa_K2185004 BBa_K392003 | BBa_K165002 MFa1-Secre BBa_K2185004 BBa_K392003 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K165002 BBa_K792002 BBa_K2185004 BBa_K392003 | 1,18 27,80 87,1727 1736,1864 | BBa_K165002 MFa1-Secre BBa_K2185004 BBa_K392003 |