BBa_K2185007

BBa_K2185007 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K2185007
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Broad Run iGEM Team
Date created: 2016-09-04 11:00:00
Date modified: 2016-09-15 01:55:03

S.ferax alpha amylase w/ cyc promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K2185007_sequence (Version 1)

Description

This composite part, designed and submitted by the 2016 Broad Run iGEM Team, contains an gene from the fungus S.ferax that codes for an alpha amylase enzyme. This composite part contains a constitutive promoter, coding sequence, kozak sequence, a mating factor secretion tag, and a terminator. The amylase enzyme can be expressed by cloning this part into a plasmid without a promoter built in (such as the S.cerevisiae vector pRS426).

Notes

Codon optimized for S.cerevisiae, EcoR1 and Spe1 sites removed.

Source

The coding sequence of this part comes from the fungus S.ferax

igem#experience
None
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K2185007/1