Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sarah Sandock
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:30
Sarcoisine Dimethyl Glycine Methyl Transferase (SDMT)
| Types | DnaRegion |
| Roles | Coding CDS |
| Sequences | BBa_K220000_sequence (Version 1) |
Description
SDMT is a plasmid gene (900 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. This enzyme of this pathway is SDMT, which stands for Sarcosine Dimethylglycine Methyl Transferase. It catalyzes two steps of methylation, both that of sarcosine and dimethylglycine, to our final product, betaine, again using SAM as the methyl donor. The gene we used came from Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. In our project, we really appreciate that Center for Eukaryotic Structural Genomics at University of Wisconsin-Madison graciously gave us the plasmid containing this gene. We PCR amplified gene SDMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.Reference: J McCoy, L Bailey, Y NG, C Bingman, R Wrobel, A Weber, B Fox, G Philips
Discovery of sarcosine dimethylglycine methyltransferase from Galdieria sulphuraria
http://www3.interscience.wiley.com/cgi-bin/fulltext/120751555/HTMLSTART
Notes
Length: 56Fwd: 5'- AAC GAA TTC TAC TCT AGA AGG AGG ATA ATT TAA TGA GAG TGG AAA ATA GCA ACG GA-3'
Length: 33
Rev: 5'- ACC ACT AGT CTA CTA TAT TTT GTC GGA CTT GCG-3'