BBa_K220002

BBa_K220002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K220002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Sandock

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:30

Glycine Sarcosine Methyl Transferase Gene

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K220002_sequence (Version 1)

Description

GSMT is a plasmid gene (854 bp) that codes for the enzyme that catalyze the first reaction in the salt tolerant pathway that we are interested. GSMT stands for Glycine Sarcosine N-Methyl Transferase. It catalyzes the conversion from glycine to sarcosine, using SAM as a methyl donor. In our project, we have this GSMT synthesized using MR. Gene. Naturally, this gene can be found in Galdieria sulphuraria, an extremephile that establishes high resistance to osmotic stress as well as acidic, thermal stresses and toxic metals. We PCR amplified gene GSMT with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Reference: A Nyyss??l??, T Reinikainen, M Leisola
Characterization of Glycine Sarcosine N-Methyltransferase and Sarcosine Dimethylglycine N-Methyltransferase
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92834/?tool=pubmed

Notes

Codon optimization and synthesis of its coding region from Ectothiorhodospira Halochloris

Source

Codon optomization and synthesis of its coding region from Ectothiorhodospira Halochloris

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K220002/1

Member of these Collections

• iGEM Parts Registry