BBa_K220004

BBa_K220004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K220004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Sarah Sandock

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:31

Salt Stress Activated Promoter Pro U

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K220004_sequence (Version 1)

Description

The ProU promoter (658 bp) was obtained from wild type K12 E. Coli Genomic DNA. Pro U controls transcription of the Pro U operon which contains three sub-units instrumental in bringing choline and proline into the cell. The promoter is most active during times of osmotic stress. We PCR amplified the gene proU with iGEM designated cutting sites and ribosome binding sites and biobricked it with pSB1A2.

Reference: T Annamalai, K Venkitanarayanan
Role of proP and proU in betaine uptake by Y. enterocolitica under cold and osmotic stress conditions
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2655450/?tool=pubmed"

Notes

Length: 30
Fwd: 5'- AAT ACT AGT CTT TTT TCT GCG CAC CTC ACG -3'

Length: 42
Rev: 5'- AAT ACT AGT GCA ATA GAA AGA TTC CTT TAT TTG TCT ATG TCG -3'

Source

Gene isolation from K12 E. Coli Genomic DNA

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K220004/1

Member of these Collections

• iGEM Parts Registry