Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Anusuya Ramasubramanian
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:31
5MT Mutant TrpR gene
| Types | DnaRegion |
| Roles | engineered_region Generator |
| Sequences | BBa_K223052_sequence (Version 1) |
Description
The 5 methyl-L-tryptophan aporepressor gene is a mutant version of the regular TrpR gene involved in sensing levels of synthetic compound, 5 MT. It works by binding to its corepressor, 5 MT, and then to its corresponding promoter operator, BBa_K223051, repressing downstream transcription. In the presence of low corepressor levels, the 5MT aporepressor will not bind allowing for downstream transcription.Notes
n/aSource
Original part taken from GenBank Escherichia coli IAI1: http://www.ncbi.nlm.nih.gov/nuccore/218552585?from=4690442&to=4690768& report=gbwithpartsTwo point mutation modifications made to change protein residue at 58 and 79 to Leu and Lys, respectively.