BBa_K228001

BBa_K228001 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228001
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Lin Min
Date created: 2009-08-24 11:00:00
Date modified: 2015-05-08 01:11:32

SupD-tRNA



    • Details

    Types
    DnaRegion

    Roles
    RNA

    mature_transcript_region

    Sequences BBa_K228001_sequence (Version 1)

    Description

    The SupD-tRNA part functions as a tRNA, it suppresses the TAG amber stop codon and translate it into a Ser. There is no need to assembly SupD-tRNA with a rbs, since it is not translated.

    This part, together with the T7ptag part, make an AND Gate, that is, in the presence of both the 2 parts, T7 polymerase is tranlated into a functional polymerase that transcrips a downstream element constructed after a T7 promoter. Which is the output of the AND Gate.

    Notes

    There is a PstI site in the original sequence, but luckily it is near the 3' end of this part, so we designed a mutation in our primer and mutated the PstI site.

    Source

    SupD-tRNA again is from Voigt Lab(UCSF), We standardlize this part by PCR strategy.

    igem#experience
    Works
     
    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K228001/1
     

    Attachments

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