BBa_K228003

BBa_K228003 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228003
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-05 11:00:00
Date modified: 2015-05-08 01:11:32

Bistable part



Types
DnaRegion

Roles
engineered_region

Device

Sequences BBa_K228003_sequence (Version 1)

Description

It is a part which has two stable states--CI and CI434 and the orginal state can be either of the two. CI repressor is expressed after a CI434 repressible promoter, while the CI434 is expressed after a CI repressible promoter, thus the system can only stay in one state, when one is expressed and the other is repressed. The two states can be swicthed in certain conditions. In other words, the orginal state can be changed by exogenetic triggering. For example, the orginal state is CI, then you can use an gene which exogenetically expresses CI434 to trigger the bistable part into the state of CI434.

Source

The part is made up of the standard parts from partsregistry, and some of the sub-parts are inverted by PCR.

Sequence Annotation Location Component / Role(s)
mRFP
eGFP
CI434 repressor
rbs
lamda CI repressor
rbs
rbs
CI regulated promoter
rbs
CI434 OR1
CI434 regulated promoter
1,681
2219,2938
1561,2196
2203,2211
707,1420
688,700
1426,1432
1491,1539
1546,1554
1439,1454
1439,1490
CDS feature/cds
feature/cds CDS
CDS feature/cds
ribosome_entry_site feature/rbs
CDS feature/cds
feature/rbs ribosome_entry_site
ribosome_entry_site feature/rbs
promoter feature/promoter
feature/rbs ribosome_entry_site
feature/binding non_covalent_binding_site
promoter feature/promoter
igem#experience
Works
 
igem#sampleStatus
It's complicated
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K228003/1