BBa_K228115

BBa_K228115 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K228115
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:32

AND GATE AraC + SupD + LacP +RBS(B0034)+T7ptag



Types
DnaRegion

Roles
Device

engineered_region

Sequences BBa_K228115_sequence (Version 1)

Description

This Device belongs to the SupD_T7ptag AND Gate family constructed by Peking University 2009 iGEM Team

This is a AND GATE in the Ecoli stain JM109. Use arabinose to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.
Note: this AND gate make use of the lacI on the F plasmid of JM109, and because of the instability of the F plasmid, the leakage of lacP is significant. For improvement, a lacI generator can be assemble into it. This gate only works on low copy plasmid, such as pSB4K5, due to the lacI on the F plasmid.
This gate is only one of nine gates(K228110-K228118), which are different in the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133

Source

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BBa_K228115/1