Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shan Shen
Date created: 2009-09-21 11:00:00
Date modified: 2015-05-08 01:11:32
AND GATE LacP+RBS(B0030)+T7ptag+LuxP+SupD
| Types | DnaRegion |
| Roles | engineered_region Device |
| Sequences | BBa_K228119_sequence (Version 1) |
Description
This is a AND GATE in the Ecoli stain JM109. Use HSL to induce the transcription of SupD and use IPTG to induce the transcription of T7ptag. If the SupD tRNA is existent, T7ptag can be translated into functional T7 polymerase, and otherwise the translation of T7ptag will come to stop because of some amber stop codon mutations in the T7ptag. The T7 polymerase will activate a T7 promoter.Note: this AND gate make use of the lacI on the F plasmid of JM109, and because of the instability of the F plasmid, the leakage of lacP is significant. For improvement, a lacI generator can be assemble into it. This gate only works on low copy plasmid, such as pSB4K5, due to the lacI on the F plasmid.
This gate is only one of nine gates(K228119-K228127), which are different in the RBS of T7ptag.
For more information: refer to K228000(T7ptag) and K228001(SupD) referrence: Anderson JC, Voigt CA, Arkin AP (2007) Environmental signal integration by a modular AND gate. Mol Syst Biol 3: 133
Source
It is made up of standard subparts from the partregistry.| Access | Instance | Definition |
| public public | BBa_K228011 BBa_K228101 | BBa_K228011 BBa_K228101 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K228011 BBa_K228101 | 1,1325 1334,4372 | BBa_K228011 BBa_K228101 |