BBa_K228101

BBa_K228101 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K228101

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shan Shen

Date created: 2009-09-07 11:00:00

Date modified: 2015-05-08 01:11:32

LacP(R0010) + RBS(B0030) + T7ptag(K228000) + terminator(B0015)

Details

• Types: DnaRegion
• Roles: engineered_region; Generator
• Sequences: BBa_K228101_sequence (Version 1)

Description

T7 polymerase with an amber mutation coding gene is constructed after Lac promoter which would be triggered by IPTG. T7 polymerase can be well expressed in the exsitence of SupD tRNA. Also, the expression of the T7 polymerase can be regulated by RBS.

Source

It is made up of standard subparts from the partregistry.

Components

Access	Instance	Definition
public	BBa_R0010	LacI
public	BBa_B0030	BBa_B0030
public	BBa_K228000	BBa_K228000
public	BBa_B0010	BBa_B0010
public	BBa_B0012	BBa_B0012

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0010	1,200	LacI
BBa_B0030	209,223	BBa_B0030
BBa_K228000	230,2902	BBa_K228000
BBa_B0010	2911,2990	BBa_B0010
BBa_B0012	2999,3039	BBa_B0012

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K228101/1

Member of these Collections

• iGEM Parts Registry