Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Guosheng Zhang
Date created: 2009-09-19 11:00:00
Date modified: 2015-05-08 01:11:33
AraC->rbs->T7ptag->Terminator
| Types | DnaRegion |
| Roles | Generator engineered_region |
| Sequences | BBa_K228213_sequence (Version 1) |
Description
This is a branch of the AND GATE built by Peking University 2009 iGEM Team. The addition of arabinose can induce the transcription of T7ptag. However, if the SupD tRNA is absent, T7ptag might not be translated into functional T7 polymerase normally because of some amber stop codon mutations in it. We also have constructed a few similar parts with various RBS, which would make the expression regulation of T7ptag gene more convenient by choosing different parts.Source
Built by joining different standardized biobricks together| Access | Instance | Definition |
| public public public public public | BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228204 | BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228204 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228204 | 47,58 1013,1092 1101,1141 1,1279 1288,4115 | BBa_B0034 BBa_B0010 BBa_B0012 BBa_K228009 BBa_K228204 |