BBa_K239002

BBa_K239002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-21 11:00:00

Date modified: 2015-05-08 01:11:36

HypB promoter, activated by Fnr during oxygen deficiency

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K239002_sequence (Version 1)

Description

Sequence contains 2 Fnr binding sites but no specific promoter sequence.
Function: Switched on during anaerobic conditions.
Area of application: Detection of when the cell is switching to anaerobic conditions.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first Fnr binding site and ends 37 pb after second Fnr binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Fnr	4,17	non_covalent_binding_site, feature/binding
Fnr	110,123	non_covalent_binding_site, feature/binding
-10	147,152	promoter, feature/promoter

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239002/1

Member of these Collections

• iGEM Parts Registry