BBa_K239002

BBa_K239002 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K239002
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Axel Nystrom
Date created: 2009-06-21 11:00:00
Date modified: 2015-05-08 01:11:36

HypB promoter, activated by Fnr during oxygen deficiency



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K239002_sequence (Version 1)

Description

Sequence contains 2 Fnr binding sites but no specific promoter sequence.
Function: Switched on during anaerobic conditions.
Area of application: Detection of when the cell is switching to anaerobic conditions.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first Fnr binding site and ends 37 pb after second Fnr binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

Sequence Annotation Location Component / Role(s)
Fnr
Fnr
-10
4,17
110,123
147,152
non_covalent_binding_site feature/binding
non_covalent_binding_site feature/binding
promoter feature/promoter
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K239002/1