BBa_K239003

BBa_K239003 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239003

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-21 11:00:00

Date modified: 2015-05-08 01:11:36

Modified HypB promoter, activated by Fnr during oxygen deficiency

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K239003_sequence (Version 1)

Description

Sequence contains 2 Fnr binding sites but no specific promoter sequence. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.

Notes

Optimisation of Fnr binding sites, modification of position 2 bp.
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed.
Sequence starts 3 bp before the first Fnr binding site and ends 35 bp after second Fnr binding site.

Source

Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
Fnr	4,17	non_covalent_binding_site, feature/binding
Fnr	110,123	feature/binding, non_covalent_binding_site

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239003/1

Member of these Collections

• iGEM Parts Registry