BBa_K239004

BBa_K239004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K239004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Axel Nystrom
Date created: 2009-06-25 11:00:00
Date modified: 2015-05-08 01:11:36

Part of narGP promoter, activated by Fnr during oxygen deficiency



Types
DnaRegion

Roles
Regulatory

promoter

Sequences BBa_K239004_sequence (Version 1)

Description

Sequence contains 1 Fnr binding site, the specific promoter sequence is not determined yet, but it is transcribed by RNAP sigma factor 70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic conditions.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the the Fnr binding site and ends 35 pb after the Fnr binding site.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

Sequence Annotation Location Component / Role(s)
Fnr
4,17
non_covalent_binding_site feature/binding
igem#sampleStatus
Not in stock
igem#status
Unavailable
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K239004/1