BBa_K239005

BBa_K239005 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239005

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-25 11:00:00

Date modified: 2015-05-08 01:11:36

NarK promoter, Fnr activated under anaerobic conditions

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K239005_sequence (Version 1)

Description

Sequence contains 2 Fnr, 1 Fis, 4 NarL and 1 IHF binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions (upregulated 100-fold). Area of application: Detection of when the cell is switching to anaerobic metabolism.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the IHF binding site and ends 6 pb after the -10 box.

Source

Genomic sequence from biocyc, Escherichia coli K-12. Native unmodified sequence.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
IHF	4,16	non_covalent_binding_site, feature/binding
-10	126,133	feature/promoter, promoter
-35	98,106	feature/promoter, promoter
Fnr	92,105	non_covalent_binding_site, feature/binding
Fis	88,106	feature/binding, non_covalent_binding_site
NarL	70,76	non_covalent_binding_site, feature/binding
NarL	34,45	non_covalent_binding_site, feature/binding
Fnr	54,67	non_covalent_binding_site, feature/binding
NarL	57,63	feature/binding, non_covalent_binding_site
NarL	47,53	feature/binding, non_covalent_binding_site

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239005/1

Member of these Collections

• iGEM Parts Registry