BBa_K239006

BBa_K239006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K239006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Axel Nystrom

Date created: 2009-06-25 11:00:00

Date modified: 2015-05-08 01:11:36

Modified NarK promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K239006_sequence (Version 1)

Description

Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.

Notes

Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.

Source

Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
-10	76,83	feature/promoter, promoter
-35	48,56	promoter, feature/promoter
Fnr	42,55	non_covalent_binding_site, feature/binding
Fis	38,56	non_covalent_binding_site, feature/binding
Fnr	4,17	non_covalent_binding_site, feature/binding
NarL	20,26	feature/binding, non_covalent_binding_site
NarL	7,13	feature/binding, non_covalent_binding_site

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K239006/1

Member of these Collections

• iGEM Parts Registry