Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Axel Nystrom
Date created: 2009-06-25 11:00:00
Date modified: 2015-05-08 01:11:36
Modified NarK promoter
| Types | DnaRegion |
| Roles | Regulatory promoter |
| Sequences | BBa_K239006_sequence (Version 1) |
Description
Sequence contains 2 Fnr (one of which is modified), 1 Fis, 2 NarL binding sites and initiates transcription by RNAP sigma-70. Function: Switched on during anaerobic conditions. Area of application: Detection of when the cell is switching to anaerobic metabolism.Notes
Detect and remove possible restriction sites: XbaI, EcoRI, PstI, SpeI, AgeI, BamHI, BglII, NogMIV, NotI and XhoI. No restriction site had to be removed. Sequence starts 3 bp before the first FNR binding site and ends 6 pb after the -10 box. One pb has been changed in order optimise the first FNR binding site.Source
Raw genomic sequence from biocyc, Escherichia coli K-12, but modified.| Sequence Annotation | Location | Component / Role(s) |
| -10 -35 Fnr Fis Fnr NarL NarL | 76,83 48,56 42,55 38,56 4,17 20,26 7,13 | feature/promoter promoter promoter feature/promoter non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site feature/binding feature/binding non_covalent_binding_site feature/binding non_covalent_binding_site |