BBa_K241001

BBa_K241001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K241001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: John Heil

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:37

phiC31 attB TT

Details

• Types: DnaRegion
• Roles: DNA; sequence_feature
• Sequences: BBa_K241001_sequence (Version 1)

Description

A recognition site for the phage integrase that belongs on the donor plasmid, used for a site specific recombination, specifically, Integrase Mediated Cassette Exchange.

Notes

Synthetic oligonucleotides do not have a free 5` phosphate. In order for the ligation of annealed linkers to the annealed att site oligos to be successful at least one set of oligos must be phosphorylated. T4 polynucleotide kinase can work for this purpose.

Source

Primers were designed with the site and amplified to create double stranded fragments with prefix and suffix.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
attB TT	1,35	feature/misc, sequence_feature
core dinucleotide	18,19	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K241001/1

Member of these Collections

• iGEM Parts Registry