Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Enrique Paz
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:37
Essential region of P4 bacteriophage genome
| Types | DnaRegion |
| Roles | Other sequence_feature |
| Sequences | BBa_K242051_sequence (Version 1) |
Description
Bacteriophage P4 genome without the non-essential region nor the integrase gene.The use of this part is similar to a plasmid. You can clone standard parts in this viral vector.
You can use this vector with a system for P4 production to encapsidate your constructions and transduce them.
Notes
We remove all forbiden restriction sites, (EcoRI,XbaI,SpeI,PstI), from P4 genome but it means remove the integrase also.For our project it was useful because removing the integrase gene we expect P4 to be maintained as a plasmid. However it could mean a reduction in the stability of the plasmid without selection.
Source
This sequence comes from P4 bacteriophage genome from positions (0 to 18)+(4090 to 11624)http://www.ncbi.nlm.nih.gov/nuccore/9627507?report=graph&from=1&to=9087