Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Jes??s Abraham Avelar Rivas
Date created: 2009-10-19 11:00:00
Date modified: 2015-05-08 01:11:37
antisense RNA targeting replication machineries of T3 and T7 bacteriophages
| Types | DnaRegion |
| Roles | RNA mature_transcript_region |
| Sequences | BBa_K242250_sequence (Version 1) |
Description
It contains two antisense RNAs targeting 5' region of genes involved in DNA replication in bacteriophages T3 and T7. The first part is targeting T3 DNA polymerase, the second is to traductionaly regulate the production of the protein gp2.5 which is a ssDNA binding protein essential for replication.Notes
We looked for any target that was mentioned as essential in the literature. We decided to attack phages in their replication process, because of the targets we found, so we reasoned that if the phage tries to replicate it???s genome the asRNA is going to block the process reducing dramatically the burst size or if the efficiency is high, there wont be any new phage.we looked for structures in RNAFold with high Gibbs energy values (the less negative, the better), we also checked that the structures were not blocking the RBS.
Source
Genomic DNA from bacteriophages T3 and T7| Sequence Annotation | Location | Component / Role(s) |
| asRNA targeting T3 DNA polymerase asRNA targeting T7 gp2.5 | 1,69 70,153 | feature/misc sequence_feature sequence_feature feature/misc |