Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nika Debeljak
Date created: 2009-09-23 11:00:00
Date modified: 2015-05-08 01:11:38
BB-NIC-II-HisN
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K245005_sequence (Version 1) |
Description
BB-NIC-II-HisN originates from BioBrick vector pSB1A2 but contains modified prefix and suffix according to assembly standard 37. T7 promoter, RBS and ATG are included in prefix and T7 terminator and STOP codon in suffix. His tag is also inserted between ATG and multiple cloning site in prefix. There are four new inner restriction sites added in multiple-cloning site (NgoMIV and AgeI in prefix; XmaI and BspEI in suffix) which enable extension of part cloned into BB-NIC-II-HisN with SG on N-terminal or TG on C-terminal; extension of linker and creating a friendly scar during parts fusion.Notes
BioBrick vector pSB1A2 was first cut with EcoRI/PstI. An insert containing modified prefix and suffix (according to assembly standard 37) was ligated in between. Subsequently a ccdB domain was inserted between AgeI and XmaI restriction sites.Source
The source is BioBrick vector pSB1A2; modified prefix and suffix sequences are of synthetic origin; ccdB originates from part BBa_P1010.| Sequence Annotation | Location | Component / Role(s) |
| modified suffix XmaI BspEI SpeI STOP T7 terminator NotI PstI pBR233 origin amp resistance marker amp promoter modified prefix EcoRI NotI T7 promoter lacO regulatory site rbs START His tag XbaI NgoMIV AgeI | 1,82 1,6 7,12 13,18 19,21 22,69 70,77 77,82 244,863 1018,1878 1920,1948 2119,2261 2119,2124 2125,2132 2133,2151 2151,2178 2208,2214 2220,2222 2226,2243 2244,2249 2250,2255 2256,2261 | sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature stop_codon feature/stop stem_loop feature/stem_loop feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc feature/cds CDS promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature promoter feature/promoter non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs start_codon feature/start tag feature/tag sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature |