Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nika Debeljak
Date created: 2009-09-24 11:00:00
Date modified: 2015-05-08 01:11:38
BB-NIC-II-NC
| Types | DnaRegion |
| Roles | plasmid_vector Plasmid_Backbone |
| Sequences | BBa_K245006_sequence (Version 1) |
Description
This plasmid backbone is the same as BBa_K245004 except that it doesn't contain His tag at all.Notes
First, vector BB-NIC-II-HisN was cut with EcoRI and XbaI and insert with appropriate part of prefix modified according to standard 37 without His tag was ligated in between.Source
The basis for BB-NIC-II-NC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.| Sequence Annotation | Location | Component / Role(s) |
| XmaI modified suffix BspEI SpeI STOP T7 terminator NotI PstI pBR233 origin amp resistance marker amp promoter EcoRI NotI T7 promoter lacO regulatory site rbs START XbaI NgoMIV AgeI modified prefix | 1,6 1,82 7,12 13,18 19,21 22,69 70,77 77,82 244,863 1018,1878 1920,1948 2119,2124 2125,2132 2133,2151 2151,2178 2208,2214 2220,2222 2223,2228 2229,2234 2235,2240 2119,2240 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/misc sequence_feature feature/stop stop_codon stem_loop feature/stem_loop sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature CDS feature/cds feature/promoter promoter sequence_feature feature/misc sequence_feature feature/misc promoter feature/promoter non_covalent_binding_site feature/binding ribosome_entry_site feature/rbs sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc |