Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Nika Debeljak
Date created: 2009-09-24 11:00:00
Date modified: 2015-05-08 01:11:38
BB-NIC-II-HisC
| Types | DnaRegion |
| Roles | Plasmid_Backbone plasmid_vector |
| Sequences | BBa_K245007_sequence (Version 1) |
Description
This plasmid backbone is the same as BBa_K245004 except that it contains His tag inserted between multiple-cloning site and stop codon in suffix instead of between start codon and multiple-cloning site in prefix as in case of BBa_K245004.Notes
Using overlapping primers and PCR ligation a fragment containing modified prefix, ccdB domain and modified suffix with His tag inserted between multiple-cloning site and stop codon was first obtained. The fragment was then ligated in pSB1A2 vector cut with EcoRI and PstISource
The basis for BB-NIC-II-HisC is vector pSB1A2. Modified prefix and suffix are of synthetic origin. ccdB originates from part BBa_P1010.| Sequence Annotation | Location | Component / Role(s) |
| XmaI BspEI SpeI His tag STOP PstI pBR233 origin amp resistance marker amp promoter T7 promoter AgeI NgoMIV XbaI EcoRI START | 1,6 7,12 13,18 19,36 37,39 40,45 207,826 981,1841 1883,1911 2088,2106 2162,2167 2156,2161 2150,2155 2082,2087 2147,2149 | feature/misc sequence_feature sequence_feature feature/misc sequence_feature feature/misc feature/tag tag feature/stop stop_codon sequence_feature feature/misc sequence_feature feature/misc feature/cds CDS feature/promoter promoter promoter feature/promoter sequence_feature feature/misc feature/misc sequence_feature feature/misc sequence_feature sequence_feature feature/misc start_codon feature/start |