Types | DnaRegion
|
Roles | Regulatory
promoter
|
Sequences | BBa_K252000_sequence (Version 1)
|
Description
MicA is a regulatory small RNA. Its standard coding sequence is ligated to the lac promoter sequence (BBa_R0010)via blunt end ligation. Blunt end is necessary for function of small RNA. Restriction enzyme used for blunt end cutting is BfrBI (ATG/CAT).
Notes
Attached to the lac promoter sequence (BBa_R0010) with a blunt end restriction site. Restriction enzyme used for blunt end cutting is BfrBI (ATG/CAT).
Source
E. coli MG-1655