PBad-clyA-

BBa_K257011 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K257011

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Soufiane Boumahdi

Date created: 2009-10-16 11:00:00

Date modified: 2015-05-08 01:11:41

PBad + clyA_CTerm + RFP_NTerm

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K257011_sequence (Version 1)

Description

This part allows us to express the fusion protein clyA-RFP. In a mutant strain which constitutively produce vesicles, the fusion protein inserted in the outer membrane of the bacteria will be incorporated, during vesiculation, into the lumen of the vesicles. The promoter pBad is inductible with L-arabinose.

Notes

The fusion protein is designed following the standard 23 in order to avoid frameshift in the sequence.

Source

It's a composite part. PBad comes from the registry (2008 distribution). ClyA_CTerm :: RFP_NTerm is the BBa_K257010.

Components

Access	Instance	Definition
public	BBa_I0500	pBad/araC
public	BBa_K257010	clyA_RFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_I0500	1,1210	pBad/araC
BBa_K257010	1219,2838	clyA_RFP

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K257011/1

Member of these Collections

• iGEM Parts Registry