FecI

BBa_K257017 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K257017

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Soufiane Boumahdi

Date created: 2009-10-17 11:00:00

Date modified: 2015-05-08 01:11:41

Fec I, part of the signal transduction pathway FecARI

Details

• Types: DnaRegion
• Roles: CDS; Coding
• Sequences: BBa_K257017_sequence (Version 1)

Description

The fec transduction pathway is unique because it allows a signal to be passed from the extracellular milieu down to the cytoplasm, thus being transmitted through both the outer and inner membrane. Briefly, the outermembrane protein FecA binds ferric citrate, change its conformation to interact with FecR which is an transmembrane protein located in the inner membrane. Then FecR is able to activate FecI an alternative sigma factor specific to Pfec.

In some variant of the system, the activated FecR homologues would repress FecI activity instead of activating it.

Notes

Fec I needs to be expressed in association with Fec R behind the Pfec promoter in order to have a functional transduction pathway.

Source

For more information, see: Gene regulation by transmembrane signaling. Volkmar Braun et al., 2006

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
good rbs	1,8	feature/rbs, ribosome_entry_site
fecI	14,532	feature/cds, CDS
double codon stop	533,538	stop_codon, feature/stop

Other Properties

• igem#experience: None
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K257017/1

Member of these Collections

• iGEM Parts Registry