Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Soufiane Boumahdi
Date created: 2009-10-17 11:00:00
Date modified: 2015-05-08 01:11:41
AIDA-I autotransporter, linker + translocator domains
| Types | DnaRegion |
| Roles | polypeptide_domain Protein_Domain |
| Sequences | BBa_K257018_sequence (Version 1) |
Description
AIDA-I is the adhesin involved in diffuse adherence of E.coli. It belongs to the monomeric autotransporter protein family of the type-V secretion pathway. AIDA-I is synthesized from the aidA gene as a 145 kDa pre-proprotein with three domains : first, an N-terminal sec-dependent signal sequence that allows transport across the inner membrane; second, a cenral domain of 100kDa, the mature AIDA-I, bearing the functional part of the protein; and finally, a C-terminal membrane-embedded domain of 45kDa, AIDAc, which is predicted to form a pore in the outer membrane and might serve as a translocation conduit for mature AIDA-I.Notes
We had to deal with considerations about the linker domain which links the translocated protein to the translocator domain of AIDA-I. In fact, this linker needs to have a minimal length so that the protein translocated would be really functional at the outer side of the outer membrane.Source
AIDA has been synthesized in vitro.| Sequence Annotation | Location | Component / Role(s) |
| AIDA_I_Cterm double codon stop Linker domain Translocator domain | 1,1320 1321,1326 1,468 469,1320 | feature/cds CDS stop_codon feature/stop sequence_feature feature/misc feature/misc sequence_feature |