Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Petros Mina
Date created: 2009-10-12 11:00:00
Date modified: 2015-05-08 01:11:42
AraC-RBS-FhuA
| Types | DnaRegion |
| Roles | Device engineered_region |
| Sequences | BBa_K259010_sequence (Version 1) |
Description
What this part gives upon expression
This part encodes for FhuA directly as it is already in frame with a pBAD/Arabinose regulated promoter. Promoter Induction can occur with 0.1% Arabinose in the culture environment.
FhuA is an E.coli Outer Membrane (OM) protein used for scavenging iron from the nutrient medium. For more information on FhuA visit the FhuA main page (
Potential uses of this Device
This part can be used also to create protein fusions by adding other proteins to it's 3' end. Protein fusion can be accomplished straightforwardly using the Bioscaffold-Linker biobricks
Protein fusions with FhuA may allow delivery of proteins to the OM of E.coli cells or inclusion into Outer Membrane Vesicles (see iGEM'09 Bristol-BCCS project).
Notes
The part was assembled using standard RFC10 assembly.Source
The arabinose promoter occurs naturally in E.coli. The RBS is designed from the Anderson family. FhuA also occurs naturally in E.coli.| Access | Instance | Definition |
| public public public public | BBa_R0081 BBa_R0080 BBa_J61100 BBa_K259000 | AraC O2 AraC BBa_J61100 fhuA |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_R0081 BBa_R0080 BBa_J61100 BBa_K259000 | 1,183 192,340 349,360 367,2613 | AraC O2 AraC BBa_J61100 fhuA |