AraC O2

BBa_R0081 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_R0081
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Sara Neves (Fighting Darwins)
Date created: 2004-01-29 12:00:00
Date modified: 2015-05-08 01:14:15

Inhibitor (AraC loop attachment with O2 site)



    • Details

    Types
    DnaRegion

    Roles
    Regulatory

    promoter

    Sequences BBa_R0081_sequence (Version 1)

    Description

    this piece of DNA contains a "dead" part of coding region for AraC, and a functional Operator2 site. when attached to the rest of the promoter region, this part allows for the binding and loop formation that would result in repression of the araC promoter

    Notes

    1) changed ATG start codon to TAG stop
    2) 6 random deletions upstream of putative araC binding sites to preserve spacing for loop back (BB ends will add 6 base pairs); spacing crucial in matching up O2 and I2 binding sites (turns of DNA helix)
    3) designed to attach to R0080 to enable all (positive and negative) regulatory functions, and to form "loop back"

    Sequence Annotation Location Component / Role(s)
    defunct araC
    stem_loop
    stem_loop
    		1,78
    22,37
    57,72
    		CDS feature/cds
    stem_loop feature/stem_loop
    stem_loop feature/stem_loop

    igem#partStatus
    Released HQ 2013
    igem#sampleStatus
    In stock
    igem#status
    Available
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_R0081/1
     

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