BBa_K259014

BBa_K259014 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K259014

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Petros Mina

Date created: 2009-10-14 11:00:00

Date modified: 2015-05-08 01:11:42

AraC-RBS-FhuA-GFP

Details

• Types: DnaRegion
• Roles: engineered_region; Composite
• Sequences: BBa_K259014_sequence (Version 1)

Description

The part was designed to assess if FhuA can be delivered to the Outer Membrane and to Outer Membrane vesicles in E.coli.

For delivery purposes please use part FhuA K259000 or AraC-RBS-FhuA K259010 and create your own protein fusions using Bioscaffolds like BpuEI-BseRI K259002

Notes

The protein fusion was manipulated on a different plasmid.

Source

The part was designed using the described biobricks.

Components

Access	Instance	Definition
public	BBa_R0081	AraC O2
public	BBa_R0080	AraC
public	BBa_J61100	BBa_J61100
public	BBa_K259000	fhuA
public	BBa_E0040	GFP

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_R0081	1,183	AraC O2
BBa_R0080	192,340	AraC
BBa_J61100	349,360	BBa_J61100
BBa_K259000	367,2613	fhuA
BBa_E0040	2620,3339	GFP

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K259014/1

Member of these Collections

• iGEM Parts Registry