BBa_K266001

BBa_K266001 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K266001

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: UNAM FCIENCIAS-CINVESTAV IRA

Date created: 2009-10-12 11:00:00

Date modified: 2015-05-08 01:11:43

Inverter TetR -> LuxR

Details

• Types: DnaRegion
• Roles: Inverter; engineered_region
• Sequences: BBa_K266001_sequence (Version 1)

Description

[[Part:BBa_J23100|BBa_J23100]] constitutive promoter directs the expression of tetracycline repressor ([[Part:BBa_C0040|TetR]]). TetR binds to [[Part:BBa_R0040|pTet]] regulatory region resulting in a negative control of the production of LuxR. The whole system acts as an Inverter of Tet system controlling LuxR expression.
TetR repression is inhibited by the addition of tetracycline or its analog, aTc.

Notes

None

Source

The biobricks BBa_K145201 and BBa_K116640 where used for this biobrick

Components

Access	Instance	Definition
public	BBa_K145201	BBa_K145201
public	BBa_K116640	BBa_K116640

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K145201	1,883	BBa_K145201
BBa_K116640	892,1890	BBa_K116640

Other Properties

• igem#experience: Works
• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K266001/1

Member of these Collections

• //chassis/prokaryote/ecoli
• //direction/forward
• //promoter
• //regulation/negative
• //rnap/prokaryote/ecoli/sigma70
• iGEM Parts Registry