BBa_K270000

BBa_K270000 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K270000

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ann Lesnefsky

Date created: 2009-09-19 11:00:00

Date modified: 2015-05-08 01:11:44

Pu Promoter

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K270000_sequence (Version 1)

Description

The Pu promoter is the upper pathway promoter for toluene degradation found on the TOL plasmid pWWO in P. putida mt-2.

Notes

The Pu promoter is not annotated on the NCBI sequence and transcribes the XylU, XylW, XylM, XylA, XylB and XylN genes. A 400 bp region between the XylU gene and the tnpA transposase was selected for amplification by colony PCR where the promoter was believed to reside.

Source

NCBI: Pseudomonas putida plasmid pWW0

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pWW0	1,137	sequence_feature, feature/misc
Pu Promoter BBa_K270000	138,338	feature/promoter, promoter
UAS	165,214	feature/misc, sequence_feature
IHF	268,283	feature/misc, sequence_feature
pWW0	339,340	sequence_feature, feature/misc

Other Properties

• igem#experience: None
• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K270000/1

Member of these Collections

• iGEM Parts Registry