BBa_K270006

BBa_K270006 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K270006

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Ann Lesnefsky

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:44

Pr Promoter

Details

• Types: DnaRegion
• Roles: promoter; Regulatory
• Sequences: BBa_K270006_sequence (Version 1)

Description

Pr is a regulatory promoter for the toluene degradation pathway on the pWW0 plasmid. This is a constituative promoter that expresses the regulatory protien XylR. When no toluene is present XylR represses Pr. When toluene is present the XylR binds with the toluene and the activity of the Pr promoter increases.

Notes

The Pr XylR basic parts were amplified together from the pWW0 plasmid using colony PCR.

Source

NCBI:Pseudomonas putida plasmid pWW0

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
pWW0	1,221	sequence_feature, feature/misc
Pr Promoter	222,421	feature/promoter, promoter
UAS	254,270	sequence_feature, feature/misc
UAS	284,300	sequence_feature, feature/misc
IHF	252,273	feature/misc, sequence_feature

Other Properties

• igem#sampleStatus: Not in stock
• igem#status: Unavailable
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K270006/1

Member of these Collections

• iGEM Parts Registry