| Types | DnaRegion
|
| Roles | engineered_region
Reporter
|
| Sequences | BBa_K274001_sequence (Version 1)
|
Description
Produces dark brown pigment output. The gene (melA) codes for a tyrosinase which produces the pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site.
Notes
The only changes to the sequence from the plasmid kindly provided by The Massachusetts Institute of Technology and Gregory Stephanopoulos was the removal of two resitrction sites, and the addition of the iGEM prefix and suffix. The two PstI restriction sites were removed by converting CAG to CAA in the second codon of the restriction site.
Source
The melA gene comes from the Rhizobium etli CFN 42 symbiotic plasmid p42d (accession number U80928). The origonal plasmid was created by Christine Nicole S. Santos and Gregory Stephanopoulos (reference: http://aem.asm.org/cgi/content/abstract/74/4/1190)
