Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuna Gould
Date created: 2009-10-07 11:00:00
Date modified: 2015-05-08 01:11:45
Vio operon
| Types | DnaRegion |
| Roles | Reporter engineered_region |
| Sequences | BBa_K274002_sequence (Version 1) |
Description
Produces a purple pigment (violacein) from L-tyrosine. The operon contains five genes (VioA-E) each with their own ribosome binding sites.Notes
We added complementary restriction sites on either side of vioC and vioD (BamHI, BglII, HindIII). This allows them to be seperately removed to produce more colours from the operon.Source
The VioA-E genes were originally from from Chromobacterium voilaceum ATCC 12472 in the pPSX vio+ plasmid. This was kindly provided by John Pemberton; Department of Microbiology and parasitology, University of Queensland, Brisbane, Australia. (Sarovich & Pemberton (2007) Plasmid 57:306-313). The codons were optimised for E. coli by DNA2.0 (Welch, Villalobos, Gustafsson and Minshull (2009) J. R. Soc. Interface)| Sequence Annotation | Location | Component / Role(s) |
| rbs vioA rbs vioB rbs vioC rbs vioD rbs vioE | 1,17 18,1274 1275,1291 1292,4294 4295,4311 4312,5607 5608,5624 5625,6752 6753,6769 6770,7345 | ribosome_entry_site feature/rbs feature/cds CDS feature/rbs ribosome_entry_site CDS feature/cds feature/rbs ribosome_entry_site feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS ribosome_entry_site feature/rbs feature/cds CDS |