BBa_K274004

BBa_K274004 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K274004

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Shuna Gould

Date created: 2009-10-18 11:00:00

Date modified: 2015-05-08 01:11:45

Vio operon ABCE

Details

• Types: DnaRegion
• Roles: engineered_region; Reporter
• Sequences: BBa_K274004_sequence (Version 1)

Description

Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction with BglII and BclI. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site.

Notes

The origonal vio operon was designed with the vioD gene flanked by complementary sticky end restriction sites for BglII and BclI. This allowed the vioD to be easily removed, leading to the production of the light green pigment.

Source

This part was made from the vio operon biobrick, part number BBa_K274002. The vioD gene has been removed by restriction digest.

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
rbs	1,17	feature/rbs, ribosome_entry_site
VioA	18,1274	CDS, feature/cds
rbs	1275,1291	ribosome_entry_site, feature/rbs
VioB	1292,4294	CDS, feature/cds
rbs	4295,4311	ribosome_entry_site, feature/rbs
VioC	4312,5607	CDS, feature/cds
rbs	5608,5624	ribosome_entry_site, feature/rbs
VioE	5625,6200	CDS, feature/cds

Other Properties

• igem#experience: Works
• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K274004/1

Member of these Collections

• iGEM Parts Registry