Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Shuna Gould
Date created: 2009-10-18 11:00:00
Date modified: 2015-05-08 01:11:45
Vio operon ABCE
| Types | DnaRegion |
| Roles | engineered_region Reporter |
| Sequences | BBa_K274004_sequence (Version 1) |
Description
Produces a light green pigment from L-tyrosine. Formed from the vio operon biobrick (BBa_K274002) with the vioD gene removed by restriction with BglII and BclI. This sequence contains four genes, vioA, vioB vioD and vioE, each preceded by their own ribosome binding site.Notes
The origonal vio operon was designed with the vioD gene flanked by complementary sticky end restriction sites for BglII and BclI. This allowed the vioD to be easily removed, leading to the production of the light green pigment.Source
This part was made from the vio operon biobrick, part number BBa_K274002. The vioD gene has been removed by restriction digest.| Sequence Annotation | Location | Component / Role(s) |
| rbs VioA rbs VioB rbs VioC rbs VioE | 1,17 18,1274 1275,1291 1292,4294 4295,4311 4312,5607 5608,5624 5625,6200 | feature/rbs ribosome_entry_site CDS feature/cds ribosome_entry_site feature/rbs CDS feature/cds ribosome_entry_site feature/rbs CDS feature/cds ribosome_entry_site feature/rbs CDS feature/cds |